|
KMID : 0545119970070060378
|
|
Journal of Microbiology and Biotechnology
1997 Volume.7 No. 6 p.378 ~ p.385
|
|
|
Expression of Latent P-Type ATPases and Their Presumptive Roles in Cell Membrane of Helicobacter pylori
|
|
Yun Soon-Kyu
Hwang Se-Young
|
|
|
Abstract
|
|
|
|
Cation motive ATPases on cell membranes of Helicobacter pylori were investigated using everted membrane vesicles. Latent ATPases could be ascertained from aggregated vesicle using N,N-dimethylformamide (DMF) and Triton X-100. By contrast, ultrasonication or chloroform treatments caused membranes to be disrupted, resulting in an alteration of sensitivities against azide or vanadate. Considerable amounts of vanadate-sensitive enzymes were identified from vesicle micelles, prepared by the dilution method. These were activated in the presence of either Ni^2+ or NH^+_4. From studies employing H. pylori intact cell systems, we found that ATPase expression of this bacterium was markedly dependent upon air composition. It was interesting that cellular expression of Ni^2+ - or NH^+_4-motive ATPases was significantly affected by extracellular pH, suggesting that these unique enzymes may physiologically he involved in cellular Ni^2+ import and NH^+_4 export, respectively
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|